Journal: PLoS ONE
Article Title: Systemic Administration of Erythropoietin Inhibits Retinopathy in RCS Rats
doi: 10.1371/journal.pone.0104759
Figure Lengend Snippet: (A–K) Immunostaining for CD34 + cells (red) on retinal wholemounts stained with isolectin B4 (IB4, green). (A–F, J) RCS rats treated with EPO. (G–I, K) RCS rats without EPO treatment. In the EPO treated retina, a large number of CD34 + cells with monocyte-like (larger arrows in C and F) and microglia-like (small arrows in F) morphology were detected in the superficial (A–F) rather than the deep (J) vascular plexuses. The arrowhead in (F) points to a CD34 + cell wrapping an injured vessel. Arrows in (I) point to degenerating (D) and severely narrowed (K) retinal capillaries in rats without EPO treatment. NT = age-matched RCS rats not treated with EPO. (L) Quantitative analysis of CD34 + cells on retinal wholemounts. * P<0.05, RCS rats treated with EPO vs RCS rats not treated group; n = 6/group. Scale bars: 100 µm in (A–C, G–K); 25 µm in (D–F).
Article Snippet: In brief, cells were Fc-blocked (BD Biosciences, catalogue#550271) with 1 μg/million cells for 15 minutes on ice, and then stained with a designated antibody panel containing CD 45 PE.Cy5 (BD Biosciences, #559135), CD31 FITC (AbD Serotec, #MCA1334F), CD 146 PE (R&D Systems, #FAB3250P) CD90.1 APC (Miltenyi Biotech, #130-094-525) and CD34 PerCp.Cy5.5 (Santa Cruz, #sc-7324).
Techniques: Immunostaining, Staining